Safety Assessment of the Ultrasound Equipment Effect on the State of Some Fish Species of the Black Sea

T. B. Sigacheva1, *, T. V. Gavruseva1, E. N. Skuratovskaya1, M. P. Kirin1, N. A. Moroz2

1 A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russia

2 «All-Russian Research Institute for Nuclear Power Plants Operation», Moskow, Russia

* e-mail: mtk.fam@mail.ru

Abstract

For the commissioning of ultrasound equipment effective for the microphytofouling control of nuclear power plant hydraulic facilities, field studies are needed to confirm its safety for aquatic organisms, in particular for fish exposed to ultrasound. The paper aims to assess the effect of ultrasound equipment (power 500 W, frequency 27 kHz, current 3 A) on the behavioral response, biochemical and histopathological parameters of some Black Sea fish species in the marine environment (Karantinnaya Bay, Black Sea). The experiment was carried out over three days. In each day the ultrasound equipment was switched on for 1 h at an exposure frequency of 27 kHz. Afterwards, the individuals were kept in tanks for another five days to assess possible delayed effects. The irritating and deterrent influences were established at a short distance (10–30 cm) from the ultrasound equipment. The most pronounced behavioral reactions were recorded in red mullet Mullus ponticus, Black Sea horse mackerel Trachurus ponticus, picarel Spicara flexuosum and common stingray Dasyatis pastinaca, the least pronounced ones were noted in European black scorpionfish Scorpaena porсus. At the same time, fish mortality was not observed in the experimental and control tanks throughout the entire experiments. There were no significant differences between the biochemical parameters in the blood serum and liver, histopathological alteration indices in liver, gills and kidneys, as well as the total indices of alterations in fish from the experimental and control tanks. The obtained results indicate that the ultrasound equipment with the defined characteristics has no negative influence on fish that allows us to recommend this equipment for the application in the technical water supply system of nuclear power plants.

Keywords

ultrasound exposure, Black Sea fish, behavioral response, survival, fish survival, biochemical parameters, histopathological parameters

Acknowledgments

The work was carried out under state assignment of IBSS “Biodiversity as the basis for the sustainable functioning of marine ecosystems, criteria and scientific principles for its conservation” № 124022400148-4.

For citation

Sigacheva, T.B., Gavruseva, T.V., Skuratovskaya, E.N., Kirin, M.P. and Moroz, N.A., 2024. Safety Assessment of the Ultrasound Equipment Effect on the State of Some Fish Species of the Black Sea. Ecological Safety of Coastal and Shelf Zones of Sea, (2), pp. 137–152.

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