Assessment of Mazut Toxicity for Embryos of Two Sea Fish Species

I. I. Rudneva

Marine Hydrophysical Institute of RAS, Sevastopol, Russia

e-mail: svg-41@mail.ru

Abstract

Shelf areas of the seas and oceans characterizing high level of bioproductivity are significantly affected by anthropogenic pollution, including oil contamination. Early developmental stages of marine organisms are very sensitive to pollutants, which generate oxidative stress in them and provoke further pathological processes. We studied the influence of mazut in a concentration of 0.01 and 0.1 ml/L on the developing embryos of two benthic fish species: peacock blenny Salaria pavo and round goby Neogobius melanosthomus in Stage VI. We studied the following biomarkers: superoxide dismutase (SOD), catalase (CAT), peroxidase (PER) and glutathione reductase (GR) spectrophotometrically. The results showed high toxicity of mazut accompanied with the changes in the activity of key antioxidant enzymes in the embryos of both tested fish species, which generated oxidative stress in developing fish exposed to mazut. The general trends and peculiarities of the responses of embryo enzymes to the oil intoxication were shown, which depended on the morphological peculiarities of eggs of the tested fish species. The peacock blenny egg has thicker shell than the round goby egg, therefore, it is protected better from the environmental impact. The paper discusses possibilities of use of the demersal fish eggs for the assessment of ecological status of shelf areas in case of oil pollution.

Keywords

Black Sea, mazut, pollution, fish embryos, biomarkers, antioxidant enzymes

Acknowledgments

This study was supported by the Russian Foundation of Basic Research (Project no. 18-44-920007 “The role of global and local factors in the formation of ichthyoplanktonic communities in the Black Sea)”. The author is grateful to Dr. T.L. Chesalina for her help in experimental design development.

For citation

Rudneva, I.I., 2022. Assessment of Mazut Toxicity for Embryos of Two Sea Fish Species. Ecological Safety of Coastal and Shelf Zones of Sea, (2), pp. 118–127.

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