Аnaerobic Bacteria in Coastal Sediments of the Troitskaya Bay (Sevastopol, the Black Sea)

N. V. Burdiyan*, S. V. Alyomov

A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russia

* e-mail: burdiyan@mail.ru

Abstract

Bacteriological analysis is considered to be the most indicative in determining the ecological status of water bodies. The land – sea contact zone, especially within port water areas, is subject to constant oil pollution. In this paper, we analyze the annual dynamics of abundance and distribution features of sulfate-reducing, thiobacteria, and denitrifying groups of bacteria in the coastal sediments of a natural beach of the Troitskaya Bay, which is exposed to a strong anthropogenic load. Sampling and subsequent treatment of the material were conducted using standard methods of hydrobiology and general microbiology. In the coastal sediments the abundance of thiobacteria, denitrifying and sulfatereducing bacteria varied from 9.5·10 to 7.5·103, from 1 to 2.5·103 and from 2.5·102 to 2.5·104 cell/g, respectively. General presence of the examined bacterial groups is proven. Seasonal differences of quantitative content of the studied bacteria groups are not noticed. The capacity to utilize oil hydrocarbons as the only carbon and energy source was tested in continuous cultures of sulfate-reducing, thiobacteria and denitrifying bacteria isolated from coastal sediments, which reflects participation of these groups of microorganisms in biodegradation of petroleum hydrocarbons in the coastal zone. The obtained results are the basis for further studies of the environmental coastal zone of Sevastopol.

Keywords

thiobacteria, sulfate-reducing, denitrifying bacteria, oil, coastal sediments, the Black Sea

Acknowledgments

The authors are grateful to Yu. V. Doroshenko, department of Marine Sanitary Hydrobiology of A.O. Kovalevsky IBSS, for assistance with sampling. The research is performed under state order “Molismologic and biogeochemical base of marine ecosystem homeostasis” of A. O. Kovalevsky IBSS (No. АААА-А18-118020890090-2) and funded by RFBR grant no. 18-44-920028 р_а “Analysis of the current state of structural and functional organization of the Sevastopol region bottom biocenoses and their resilience to natural and anthropogenic factors variability”.

For citation

Burdiyan, N.V. and Alyomov, S.V., 2020. Аnaerobic Bacteria in Coastal Sediments of the Troitskaya Bay (Sevastopol, the Black Sea). Ecological Safety of Coastal and Shelf Zones of Sea, (2), pp. 122–130. doi:10.22449/2413-5577-2020-2-122-130 (in Russian).

DOI

10.22449/2413-5577-2020-2-122-130

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