Photosynthetic Parameters of Phytoplankton and Primary Production in Coastal Waters of the Black Sea: Seasonal Features

D. V. Kalmykova, T. Ya. Churilova, T. V. Efimova*, N. A. Moiseeva, E. Yu. Skorokhod

A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russia

* e-mail: tefimova@ibss-ras.ru

Аннотация

This paper studies seasonal variability of phytoplankton photosynthetic parameters in the Black Sea coastal waters near the Crimean Peninsula. Primary production was estimated using a full spectral approach that took into account phytoplankton spectral light absorption coefficient, spectral downwelling irradiance and quantum yield of photosynthesis. In the coastal waters near the village of Katsiveli, the annual dynamics of chlorophyll a concentration was characterized by maximal values in winter and minimal (half as many) in summer, while in the waters near Sevastopol, the maximum was recorded in early October and March, which is likely due to the “bloom” of phytoplankton. High annual variability of both the phytoplankton spectrally weighted chlorophyll a specific absorption coefficient (~ 3 times) and maximum quantum yield of photosynthesis (~ 7 times) was noted, with maximal values in summer and winter, respectively. In the vertical profile, the minimal values of the quantum yield were observed in the sea surface layer associated with the high photosynthetically active radiation incident on the sea surface. The variability in photosynthetic parameters, caused by phytoplankton adaptation to changing environmental factors, resulted in a strong annual variability of primary production, which varied ~ 8-fold over the study period. The obtained patterns of variability of the basic photosynthetic parameters of phytoplankton (the ability to absorb light quanta and use them in photochemical reactions) are necessary for understanding the mechanism of formation of primary production in the studied water region.

Ключевые слова

chlorophyll a, quantum yield of photosynthesis, light absorption, photosynthetically available radiation, spectral downwelling irradiance

Благодарности

We thank E. A. Zemlyanskaya for her efficient contribution to the sample treatments and E. А. Khvalkov for assistance with translation. The present study was carried out within the framework of state assignment no. 124030100106-2 “Study of regional bio-optical properties for development of satellite algorithm for assessment of multi-scale variability of primary production characteristics of pelagic ecosystems”.

Информация об авторах

Daria V. Kalmykova, Junior Researcher, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2, Nakhimov Ave., Sevastopol, 299011, Russia), Laboratory of Photosynthesis and Marine Bio-optics, ORCID ID: 0000-0002-3097-9913, Scopus Author ID: 58688288100, SPIN-code: 9699-8087, daria-k.kalmykova@ibss-ras.ru

Tanya Ya. Churilova, Senior Researcher, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2, Nakhimov Ave., Sevastopol, 299011, Russia), Laboratory of Photosynthesis and Marine Bio-optics, PhD (Biol.), ORCID ID: 0000-0002-0045-7284, Scopus Author ID: 6603622802, ResearcherID: O-8437-2016, SPIN-code: 2238-9533, tanya.churilova@ibss-ras.ru

Tatiana V. Efimova, Senior Researcher, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2, Nakhimov Ave., Sevastopol, 299011, Russia), Head of Laboratory of Photosynthesis and Marine Bio-optics, PhD (Biol.), ORCID ID: 0000-0003-3908-4160, Scopus Author ID: 57194423783, ResearcherID: X-1355-2019, SPIN-code: 1668-0742, tefimova@ibss-ras.ru

Nataliia A. Moiseeva, Researcher, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2, Nakhimov Ave., Sevastopol, 299011, Russia), Laboratory of Photosynthesis and Marine Bio-optics, SPIN-code: 8946-3315, ORCID ID: 0000-0003-1356-7981, moiseeva@ibss-ras.ru

Elena Yu. Skorokhod, Researcher, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2, Nakhimov Ave., Sevastopol, 299011, Russia) Laboratory of Photosynthesis and Marine Bio-optics, SPIN-code: 3314-5775, ORCID ID: 0000-0002-3057-3964, elenaskorokhod@ibss-ras.ru

Для цитирования

Kalmykova D. V., Churilova T. Ya., Efimova T. V., Moiseeva N. A. et al. Photosynthetic parameters of phytoplankton and primary production in coastal waters of the Black Sea: seasonal features // Экологическая безопасность прибрежной и шельфовой зон моря. 2026. № 2. С. 124–144. EDN KIQVDM.

Kalmykova, D.V., Churilova, T.Ya., Efimova, T.V., Moiseeva, N.A. and Skorokhod, E.Yu., 2026. Photosynthetic Parameters of Phytoplankton and Primary Production in Coastal Waters of the Black Sea: Seasonal Features. Ecological Safety of Coastal and Shelf Zones of Sea, (2), pp. 124–144.

Список литературы

  1. Falkowski P. G., Knoll A. H. Evolution of primary producers in the sea. Academic Press, 2011. 456 p.
  2. Falkowski P. G, Raven J. A. Aquatic photosynthesis. Third edition. New Jersey: Princeton University Press, 2007. 501 p.
  3. Shalovenkov N. Scales of ecological processes and anthropogenous loads on the coastal ecosystems of the Black Sea // Estuarine, Coastal and Shelf Science. 2000. Vol. 50, iss.1. P. 11–16.
  4. Cociasu A., Dorogan L., Humborg C., Popa L. Longterm ecological changes in Romanian coastal waters of the Black Sea // Marine Pollution Bulletin. 1996. Vol. 32(1). P. 32–38. https://doi.org/10.1016/0025-326X(95)00106-W
  5. Lillebø A. I., Stålnacke P., Gooch, G. D. Coastal lagoons in Europe: integrated water resource strategies. London : IWA Publishing, 2015. https://doi:10.2166/9781780406299
  6. Kirk J. Light and photosynthesis in aquatic ecosystems. UK, Cambridge University Press, 2011. 524 p.
  7. Morel A. Light and marine photosynthesis: a spectral model with geochemical and climatological implications // Progress in Oceanography. 1991. Vol. 26. P. 263–306. https://doi.org/10.1016/0079-6611(91)90004-6
  8. Smyth T. J., Tilstone G. H., Groom S. B. Integration of radiative transfer into satellite models of ocean primary production // Journal of Geophysical Research: Oceans. 2005. Vol. 110(C10). C10014. https://doi.org/10.1029/2004JC002784
  9. Чурилова Т. Я., Суслин, В. В., Кривенко О. В., Ефимова Т. В. и др. Спектральный подход к оценке скорости фотосинтеза фитопланктона в Черном море по спутниковой информации: методологические аспекты развития региональной модели // Журнал Сибирского федерального университета. Биология. 2016. Т. 9, № 4. P. 367–384. https://doi:10.17516/1997-1389-2016-9-4-367-384.
  10. Churilova T., Suslin V. Parameterization of light absorption by all in-water optically active components in the Black Sea: Impact for underwater irradiance and primary production modelling // Proceedings of the fifth international conference on EuroGOOS: Coastal to Global Operational Oceanography: Achievements and Challenges. 20–22 May 2008. Exeter, UK. EuroGOOS Office, 2010. Vol. 28. P. 199–205.
  11. Чурилова T. Я., Кривенко О. В., Суслин В. В., Ефимова Т. В. и др. Первичная продукция Черного моря: спектральный подход // Морской биологический журнал. 2016. Т. 1, № 3. С. 50–53. https://doi.org/10.21072/mbj.2016.01.3.08
  12. Иванов В. А., Белокопытов В. Н. Океанография Черного моря. Севастополь : ЭКОСИ-Гидрофизика, 2011. 209 с.
  13. Jeffrey S. W., Humphrey G. F. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton // Biochemie und Physiologie der Pflanzen. 1975. Vol. 167, iss. 2, P. 191–194. https://doi.org/10.1016/s0015-3796(17)30778-3
  14. Lorenzen C. J. Determination of chlorophyll and pheopigments: spectrophotometric equations // Limnology and Oceanography. 1967. Vol. 12. P. 343–346. https://doi.org/10.4319/lo.1967.12.2.0343
  15. Neeley A. R., Mannino A., eds. Inherent Optical Property Measurements and Protocols: Absorption Coefficient. Dartmouth, NS, Canada, 2018. 83 p. (IOCCG Protocol Series Ocean Optics & Biogeochemistry Protocols for Satellite Ocean Colour Sensor Validation; vol. 1). https://doi.org/10.25607/OBP-119
  16. Mannino A., Novak M. G., Nelson N. B., Belz M. et al. Measurement protocol of absorption by chromophoric dissolved organic matter (CDOM) and other dissolved materials // Inherent Optical Property Measurements and Protocols: Absorption Coefficient / A. Mannino, M. G. Novak (eds.). Dartmouth, NS, Canada : IOCCG, 2019. 77 p. (IOCCG Ocean Optics and Biogeochemistry Protocols for Satellite Ocean Colour Sensor Validation ; vol. 5.0).
  17. Marra J., Trees C. C., Bidigare R. R., Barber R. T. Pigment absorption and quantum yields in the Arabian Sea // Deep Sea Research. Part II: Topical Studies in Oceanography. 2000. Vol. 47, iss. 7–8. P. 1279–1299. https://doi.org/10.1016/S0967-0645(99)00144-7
  18. Lawrenz E., Silsbe G., Capuzzo E., Ylöstalo P. et al. Predicting the electron requirement for carbon fixation in seas and oceans // PLoS ONE. 2013. Vol. 8, iss. 3. e58137. https://doi.org/10.1371/journal.pone.0058137
  19. Antal T., Konyukhov I., Volgusheva A., Plyusnina T. et al. Chlorophyll fluorescence induction and relaxation system for the continuous monitoring of photosynthetic capacity in photobioreactors // Physiologia Plantarum. 2019. Vol. 165, iss. 3. P. 476–486. https://doi.org/10.1111/ppl.12693
  20. Zhu Y., Ishizaka J., Tripathy S. C., Wang S. et al. Relationship between light, community composition and the electron requirement for carbon fixation in natural phytoplankton // Marine Ecology Progress Series. 2017. Vol. 580. P. 83–100. https://doi.org/10.3354/meps12310
  21. Cruz S., Serôdio J. Relationship of rapid light curves of variable fluorescence to photoacclimation and non-photochemical quenching in a benthic diatom // Aquatic Botany. 2008. Vol. 88, iss. 3. P. 256–264. https://doi.org/10.1016/j.aquabot.2007.11.001
  22. Platt T., Gallegos C. L., Harrison W. G. Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton // Journal of Marine Research. 1980. Vol. 38. P. 687–701.
  23. Babin M., Morel A., Claustre H., Bricaud A. et al. Nitrogen-and irradiance-dependent variations of the maximum quantum yield of carbon fixation in eutrophic, mesotrophic and oligotrophic marine systems // Deep-Sea Research. Part I: Oceanographic Research Papers. 1996. Vol. 43, iss. 8. P. 1241–1272.
  24. Churilova T. Ya., Suslin V. V., Sosik H. M. Dependence of the photosynthetic quantum yield on phytoplankton light absorption: equations for assessing primary production in the Black Sea // Physical Oceanography. 2021. Vol. 28, iss. 1. P. 67–77. https://doi.org/10.22449/1573-160X-2021-1-67-77
  25. Suslin V. V., Korolev S. N., Kucheryaviy A. A., Churilova T. Ya. et al. Photosynthetically available radiation on surface of the Black Sea based on ocean color data // Proceedings of SPIE. SPIE, 2015. Vol. 9680 : 21st international symposium atmospheric and ocean optics: atmospheric physics. 96800T. https://doi.org/10.1117/12.2203660
  26. de Boyer Montégut C., Madec G., Fischer A. S., Lazar A., et al. Mixed layer depth over the global ocean: An examination of profile data and a profile-based climatology // Journal of Geophysical Research: Oceans. 2004. Vol. 109, iss. C12. C12003. https://doi.org/10.1029/2004JC002378
  27. Becker V., Huszar V. L. M., Crossetti L. O. Responses of phytoplankton functional groups to the mixing regime in a deep subtropical reservoir // Hydrobiologia. 2009. Vol. 628. P. 137–151. https://doi.org/10.1007/s10750-009-9751-7
  28. Иванов В. А., Михайлова Э. Н. Апвеллинг в Черном море. Севастополь : ЭКОСИ-Гидрофизика, 2008. 92 с.
  29. Шокурова И. Г., Пластун Т. В., Касьяненко Т. Е., Станичная Р. Р. и др. Ветровые условия возникновения апвеллингов в районе Южного берега Крыма / И. Г. Шокурова [и др.] // Морской гидрофизический журнал. 2023. Т. 39, № 4. С. 435–447. EDN HWSWUX.
  30. Stramski D., Kiefer D. A. Light scattering by microorganisms in the open ocean // Progress in Oceanography. 1991. Vol. 28, iss. 4. P. 343–383. https://doi.org/10.1016/0079-6611(91)90032-h
  31. Берсенева Г. П., Чурилова Т. Я., Георгиева Л. В. Сезонная изменчивость хлорофилла и биомассы фитопланктона в западной части Черного моря // Океанология. 2004. Т. 44, № 3. С. 389–398.
  32. Финенко З. З., Суслин В. В., Ковалева И. В. Сезонные и многолетние изменения концентрации хлорофилла в Черном море по спутниковым наблюдениям // Океанология. 2014. Т. 54, № 5. С. 635–645. https://doi.org/10.7868/S00030157414050062
  33. Финенко З. З., Стельмах Л. В., Мансурова И. М., Георгиева Е. Ю. и др. Сезонная динамика структурных и функциональных показателей фитопланктонного сообщества в Севастопольской бухте // Системы контроля окружающей среды. 2017. Вып. 9(29). С. 73–82.
  34. Финенко З. З., Крупаткина Д. К. Первичная продукция в Черном море в зимне-весенний период // Океанология. 1993. Т. 33, № 1. С. 97–104. EDN YTJMZX.
  35. Morel A., Bricaud A. Theoretical results concerning light absorption in a discrete medium, and application to specific absorption of phytoplankton // Deep Sea Research. Part A: Oceanographic Research Papers. 1981. Vol. 28, iss. 11. P. 1375–1393. https://doi.org/10.1016/0198-0149(81)90039-x
  36. Чурилова Т. Я., Финенко З. З., Акимов А. И. Пигменты микроводорослей // Микроводоросли Черного моря: проблемы сохранения биоразнообразия и биотехнологического использования. Севастополь : ЭКОСИ-Гидрофизика, 2008. Гл. 11. С. 301–319. EDN DCGZXE.
  37. Churilova T. Ya., Suslin V., Krivenko O. V., Efimova T. et al. Light absorption by phytoplankton in the upper mixed layer of the Black Sea: Seasonality and parametrization // Frontiers in Marine Science. 2017. Vol. 4. 90. https://doi.org/10.3389/fmars.2017.00090
  38. Raven J. A. The cost of photoinhibition // Physiologia Plantarum. 2011. Vol. 142, iss. 1. P. 87–104. https://doi.org/10.1111/j.1399-3054.2011.01465.x
  39. Emerson R., Lewis C. M. The dependence of the quantum yield of Chlorella photosynthesis on the wavelength of light // American Journal of Botany. 1943. Vol. 30, iss. 3. P. 165–178. https://doi.org/10.2307/2437236 30:165–178
  40. Woźniak B., Ficek D., Ostrowska M., Majchrowski R. et al. Quantum Yield of Photosynthesis in the Baltic: a New Mathematical Expression for Remote Sensing Applications // Oceanologia. 2007. Vol. 49, iss. 4. P. 527–542.
  41. Babin M. Phytoplankton fluorescence: theory, current literature and in situ measurement // Real-time Coastal Observing Systems for Marine Ecosystem Dynamics and Harmful Algal Blooms: Theory, Instrumentation and Modelling / M. Babin, C. S. Roesler, J. J. Cullen (eds.). Paris : UNESCO Publishing, 2008. P. 237–280.
  42. Falkowski P. G. Light-shade adaptation and assimilation numbers // Journal of Plankton Research. 1981. Vol. 3, iss. 2. P. 203–216. https://doi.org/10.1093/plankt/3.2.203
  43. Cullen J. J., Lewis M. R. The kinetics of algal photoadaptation in the context of vertical mixing // Journal Plankton Research. 1988. Vol. 10, iss. 5. P. 1039–1063. https://doi.org/10.1093/plankt/10.5.1039
  44. Prézelin B. B., Putt M., Glover H. E. Diurnal patterns in photosynthetic capacity and depth-dependent photosynthesis-irradiance relationships in Synechococcus spp. and larger phytoplankton in three water masses in the Northwest Atlantic Ocean // Marine Biology. 1986. Vol. 91. P. 205–217. https://doi.org/10.1007/BF00569436
  45. Marra J., Chamberlain W. S., Knudson C. Proportionality between in situ carbon assimilation bio-optical measures of primary production in the Gulf of Maine in summer // Limnology and Oceanography. 1993. Vol. 38, iss. 1. P. 232–238. https://doi.org/10.4319/lo.1993.38.1.0232
  46. Mikaelyan A. S., Sergeeva A. V., Pautova L. A., Chasovnikov V. K. et al. 75-Year dynamics of the Black Sea phytoplankton in association with eutrophication and climate change // Science of the Total Environment. 2024. Vol. 954. 176448. https://doi.org/10.1016/j.scitotenv.2024.176448

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