<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="research-article" dtd-version="1.3" xml:lang="en">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">Ecological Safety of Coastal and Shelf Zones of Sea</journal-id>
      <journal-title-group>
        <journal-title>Ecological Safety of Coastal and Shelf Zones of Sea</journal-title>
      </journal-title-group>
      <issn publication-format="print">2413-5577</issn>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">20240407</article-id>

      <article-categories>
        <subj-group subj-group-type="article-type">
          <subject>Research Article</subject>
        </subj-group>
      </article-categories>

      <title-group>
        <article-title>The Ability to Accumulate and Transform Diesel Fuel by Green Algae Ulva lactuca of the Barents Sea</article-title>
      </title-group>

      <contrib-group>
        <contrib contrib-type="author">
          <name>
            <surname>Voskoboiniakov</surname>
            <given-names>G. M.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>grvosk@mail.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Metelkova</surname>
            <given-names>L. O.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>Larissa.metelkova@list.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Salakhov</surname>
            <given-names>D. O.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>Salahov04@yandex.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Kudryavtsteva</surname>
            <given-names>E. O.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>ekato393@mail.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
      </contrib-group>

      <aff id="aff1">
        <institution>Murmansk Marine Biological Institute of RAS</institution>
        <addr-line>Murmansk</addr-line>
        <country country="RU">Russia</country>
      </aff>

      <pub-date date-type="pub" iso-8601-date="2024-12-20" publication-format="electronic">
        <day>20</day>
        <month>12</month>
        <year>2024</year>
      </pub-date>
      <volume>2024</volume>
      <issue>4</issue>
      <fpage>95</fpage>
      <lpage>105</lpage>

      <history>
        <date date-type="received" iso-8601-date="2024-06-03">
          <day>03</day>
          <month>06</month>
          <year>2024</year>
        </date>
        <date date-type="rev-recd" iso-8601-date="2024-09-18">
          <day>18</day>
          <month>09</month>
          <year>2024</year>
        </date>
        <date date-type="accepted" iso-8601-date="2024-06-23">
          <day>23</day>
          <month>06</month>
          <year>2024</year>
        </date>
      </history>

      <permissions>
        <copyright-statement>Copyright © 2024, G. M. Voskoboiniakov, L. O. Metelkova, D. O. Salakhov, E. O. Kudryavtsteva</copyright-statement>
        <copyright-year>2024</copyright-year>
        <copyright-holder>G. M. Voskoboiniakov, L. O. Metelkova, D. O. Salakhov, E. O. Kudryavtsteva</copyright-holder>
        <ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/>
        <license>
          <ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://creativecommons.org/licenses/by-nc/4.0/</ali:license_ref>
        </license>
      </permissions>
      <self-uri xlink:href="https://ecological-safety.ru/en/repository/issues/2024/04/07/" xlink:title="Page of article">https://ecological-safety.ru/en/repository/issues/2024/04/07/</self-uri>

      <abstract>
        <p>The article presents the results of experiments on the ability of the green alga Ulva lactuca to absorb and transform diesel fuel from marine water for 5 and 10 days. The original marine water contained 0.62 mg/L of petroleum hydrocarbons, which is about 12 maximum permissible concentrations (MPC). During the experiment with the addition of 20 mg/L of diesel fuel (400 MPC) to the water, the absorption of the introduced hydrocarbons was observed in the experimental tanks without algae. Apparently, they were absorbed by water microorganisms. On the 5th day of the experiment, the petroleum hydrocarbons concentration in the water decreased by 40% and amounted to 12 mg/L (240 MPC). When ulva thalli were added to the water, the total content of petroleum hydrocarbons in the water on the 5th day decreased by 86% (to 2.8 mg/L), and on the 10th day, it increased (to 4.2 mg/L). A slight increase in the concentration of diesel fuel hydrocarbons in water indicates a reverse process of releasing hydrocarbons absorbed by ulva into water. In the experiment with the addition of diesel fuel to the water at a concentration of 10 mg/L, the content of hydrocarbons in algae tissues on the 5th and 10th days was recorded at the level of 0.6 mg/g. The marker ratio of Σ n-alkanes / Σ petroleum products in ulva during the experiment was 0.2. A decrease in this indicator to 0.18 on the 10th day of the experiment indicates the beginning of the transformation of the hydrocarbons chemical structure. When 20 mg/L of diesel fuel (400 MPC) were added to the water, this indicator on the 5th and 10th days was 0.25 and 0.28, respectively, indicating an active process of hydrocarbon absorption by the algae surface, which was not yet complete by the 10th day. The experiment results allow us to conclude that U. lactuca is able to absorb and transform petroleum hydrocarbons and participates in the bioremediation of coastal waters.</p>
      </abstract>

      <kwd-group>
        <kwd>Ulva lactuca</kwd>
        <kwd>Barents Sea</kwd>
        <kwd>diesel fuel</kwd>
        <kwd>petroleum product accumulation</kwd>
        <kwd>petroleum product destruction</kwd>
        <kwd>pollution tolerance</kwd>
      </kwd-group>

      <funding-group>
        <funding-statement>The work was funded by the Russian Science Foundation under grant 22-17-00243 "Radiation oceanology and geoecology of the coastal shelf of the Barents and White Seas. Bio-abiotic interactions in the system: bottom sediments-water-macroalgae-microorganisms, their role in remediation of the marine coastal ecosystem under radiation and chemical contamination in the Arctic conditions".</funding-statement>
      </funding-group>

      <author-notes>
        <p><bold>About the authors:</bold></p>
        <p><bold>Grigory M. Voskoboinikov</bold>, Head of Laboratory, Murmansk Marine Biological Institute of RAS (17 Vladimirskaya Str., Murmansk, 183038, Russian Federation), DSc (Biol.), Professor, ResearcherID: G-4094-2016, Scopus Author ID: 7004206680, <email>grvosk@mail.ru</email></p>
        <p><bold>Larisa O. Metelkova</bold>, Senior Research Associate, Murmansk Marine Biological Institute of RAS (17 Vladimirskaya Str., Murmansk, 183038, Russian Federation), PhD (Chem.), <email>Larissa.metelkova@list.ru</email></p>
        <p><bold>Dmitry O. Salakhov</bold>, Junior Research Associate, Murmansk Marine Biological Institute of RAS (17 Vladimirskaya Str., Murmansk, 183038, Russian Federation), <email>Salahov04@yandex.ru</email></p>
        <p><bold>Ekaterina O. Kudryavtsteva</bold>, Senior, Murmansk Marine Biological Institute of RAS (17 Vladimirskaya Str., Murmansk, 183038, Russian Federation), <email>ekato393@mail.ru</email></p>
        <p><bold>Contribution of the authors:</bold></p>
        <p><bold>Grigory M. Voskoboinikov</bold> – ideology and statement of the study problem, electron microscopic work, writing the article, editing the text</p>
        <p><bold>Larisa O. Metelkova</bold> – analytical studies of the content, transformation of diesel fuel in water and algae, analysis and discussion of the results, participation in the preparation of the article for publication</p>
        <p><bold>Dmitry O. Salakhov</bold> – participation in setting up experiments, selection of plant material, analytical studies, analysis and discussion of the results, preparation of text and graphic materials</p>
        <p><bold>Ekaterina O. Kudryavtsteva</bold> – participation in setting up experiments, sampling of water and algae, electron microscopic work, analysis and discussion of the results</p>
        <p><italic>All the authors have read and approved the final manuscript.</italic></p>
      </author-notes>
    </article-meta>
  </front>

  <body>
    <p>Article text not included.</p>
  </body>

  <back>
    <ref-list>
      <title>REFERENCES</title>
      <ref id="B1">
        <label>1.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Semanova</surname><given-names>E.V.</given-names></name>
            <name><surname>Shlykova</surname><given-names>D.S.</given-names></name>
            <name><surname>Semenov</surname><given-names>A.M.</given-names></name>
            <name><surname>Ivanov</surname><given-names>M.N.</given-names></name>
            <name><surname>Shelyakov</surname><given-names>O.V.</given-names></name>
            <name><surname>Netrusov</surname><given-names>A.M.</given-names></name>
          </person-group>
          <article-title>Bacteria-Epiphytes of Brown Macro Alga in Utilization of Oil in Ecosystems of North Sea</article-title>
          <source>Vestnik Moskovskogo Universiteta. Seriya 16. Biologiya</source>
          <year>2009</year>
          <issue>3</issue>
          <fpage>18</fpage>
          <lpage>22</lpage>
          <comment>(in Russian)</comment>
        </element-citation>
      </ref>
      <ref id="B2">
        <label>2.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Pugovkin</surname><given-names>D.V.</given-names></name>
            <name><surname>Liaimer</surname><given-names>A.</given-names></name>
            <name><surname>Jensen</surname><given-names>J.B.</given-names></name>
          </person-group>
          <article-title>Epiphytic Bacterial Communities of the Alga Fucus vesiculosus in Oil-Contaminated Water Areas of the Barents Sea</article-title>
          <source>Doklady Biological Sciences</source>
          <year>2016</year>
          <volume>471</volume>
          <fpage>269</fpage>
          <lpage>271</lpage>
          <pub-id pub-id-type="doi">10.1134/S0012496616060053</pub-id>
        </element-citation>
      </ref>
      <ref id="B3">
        <label>3.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Malavenda</surname><given-names>S.V.</given-names></name>
            <name><surname>Shoshina</surname><given-names>E.V.</given-names></name>
            <name><surname>Kapkov</surname><given-names>V.I.</given-names></name>
          </person-group>
          <article-title>Species Diversity of Seaweeds in Different Areas of the Barents Sea</article-title>
          <source>Vestnik of MSTU</source>
          <year>2017</year>
          <volume>20</volume>
          <issue>2</issue>
          <fpage>336</fpage>
          <lpage>351</lpage>
          <pub-id pub-id-type="doi">10.21443/1560-9278-2017-20-2-336-351</pub-id>
          <comment>(in Russian)</comment>
        </element-citation>
      </ref>
      <ref id="B4">
        <label>4.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Wrabel</surname><given-names>M.L.</given-names></name>
            <name><surname>Peckol</surname><given-names>P.</given-names></name>
          </person-group>
          <article-title>Effects of Bioremediation on Toxicity and Chemical Composition of No. 2 Fuel Oil: Growth Responses of the Brown Alga Fucus vesiculosus</article-title>
          <source>Marine Pollution Bulletin</source>
          <year>2000</year>
          <volume>40</volume>
          <issue>2</issue>
          <fpage>135</fpage>
          <lpage>139</lpage>
          <pub-id pub-id-type="doi">10.1016/S0025-326X(99)00181-2</pub-id>
        </element-citation>
      </ref>
      <ref id="B5">
        <label>5.</label>
        <element-citation publication-type="paper-conference">
          <person-group person-group-type="author">
            <name><surname>Liu</surname><given-names>Y.X.</given-names></name>
            <name><surname>Liu</surname><given-names>Y.</given-names></name>
            <name><surname>Lou</surname><given-names>Y.D.</given-names></name>
            <name><surname>Li</surname><given-names>N.</given-names></name>
          </person-group>
          <article-title>Toxic Effect of Oil Spill on the Growth of Ulva pertusa by Stable Isotope Analysis</article-title>
          <source>IOP Conference Series: Earth and Environmental Science. The 5th International Conference on Water Resource and Environment (WRE 2019)</source>
          <publisher-loc>Macao, China</publisher-loc>
          <year>2019</year>
          <volume>344</volume>
          <fpage>012062</fpage>
          <pub-id pub-id-type="doi">10.1088/1755-1315/344/1/012062</pub-id>
        </element-citation>
      </ref>
      <ref id="B6">
        <label>6.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Pilatti</surname><given-names>F.K.</given-names></name>
            <name><surname>Ramlov</surname><given-names>F.</given-names></name>
            <name><surname>Schmidt</surname><given-names>E.C.</given-names></name>
            <name><surname>Kreusch</surname><given-names>M.</given-names></name>
            <name><surname>Pereira</surname><given-names>D.T.</given-names></name>
            <name><surname>Costa</surname><given-names>C.</given-names></name>
            <name><surname>de Oliveira</surname><given-names>E.R.</given-names></name>
            <name><surname>Bauer</surname><given-names>C.M.</given-names></name>
            <name><surname>Rocha</surname><given-names>M.</given-names></name>
          </person-group>
          <article-title>In vitro Exposure of Ulva lactuca Linnaeus (Chlorophyta) to Gasoline–Biochemical and Morphological Alterations</article-title>
          <source>Chemosphere</source>
          <year>2016</year>
          <volume>156</volume>
          <fpage>428</fpage>
          <lpage>437</lpage>
          <pub-id pub-id-type="doi">10.1016/j.chemosphere.2016.04.126</pub-id>
        </element-citation>
      </ref>
      <ref id="B7">
        <label>7.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>El Maghraby</surname><given-names>D.</given-names></name>
            <name><surname>Hassan</surname><given-names>I.</given-names></name>
          </person-group>
          <article-title>Photosynthetic and Biochemical Response of Ulva lactuca to Marine Pollution by Polyaromatic Hydrocarbons (PAHs) Collected from Different Regions in Alexandria City, Egypt</article-title>
          <source>Egyptian Journal of Botany</source>
          <year>2021</year>
          <volume>61</volume>
          <issue>2</issue>
          <fpage>467</fpage>
          <lpage>478</lpage>
          <pub-id pub-id-type="doi">10.21608/ejbo.2021.37571.1531</pub-id>
        </element-citation>
      </ref>
      <ref id="B8">
        <label>8.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Salakhov</surname><given-names>D.O.</given-names></name>
            <name><surname>Voskoboinikov</surname><given-names>G.M.</given-names></name>
            <name><surname>Ryzhik</surname><given-names>I.V.</given-names></name>
          </person-group>
          <article-title>The Influence of Diesel Fuel on the Growth of Plants of Ulva lactuca L. (Chlorophyta) of the Barents Sea</article-title>
          <source>Nauka Yuga Rossii = Science in the South of Russia</source>
          <year>2020</year>
          <volume>16</volume>
          <issue>1</issue>
          <fpage>55</fpage>
          <lpage>59</lpage>
          <pub-id pub-id-type="doi">10.7868/S25000640200107</pub-id>
          <comment>(in Russian)</comment>
        </element-citation>
      </ref>
      <ref id="B9">
        <label>9.</label>
        <element-citation publication-type="paper-conference">
          <person-group person-group-type="author">
            <name><surname>Salakhov</surname><given-names>D.</given-names></name>
            <name><surname>Pugovkin</surname><given-names>D.</given-names></name>
            <name><surname>Ryzhik</surname><given-names>I.</given-names></name>
            <name><surname>Voskoboinikov</surname><given-names>G.</given-names></name>
          </person-group>
          <article-title>The Changes in the Morpho-Functional State of the Green Alga Ulva intestinalis L. in the Barents Sea under the Influence of Diesel Fuel</article-title>
          <source>IOP Conference Series: Earth and Environmental Science</source>
          <year>2021</year>
          <volume>937</volume>
          <issue>2</issue>
          <fpage>022059</fpage>
          <pub-id pub-id-type="doi">10.1088/1755-1315/937/2/022059</pub-id>
        </element-citation>
      </ref>
      <ref id="B10">
        <label>10.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Malavenda</surname><given-names>S.</given-names></name>
            <name><surname>Makarov</surname><given-names>M.</given-names></name>
            <name><surname>Ryzhik</surname><given-names>I.</given-names></name>
            <name><surname>Mityaeva</surname><given-names>M.</given-names></name>
            <name><surname>Malavenda</surname><given-names>S.</given-names></name>
          </person-group>
          <article-title>Occurrence of Ulva lactuca L. 1753 (Ulvaceae, Chlorophyta) at the Murman Coast of the Barents Sea</article-title>
          <source>Polar Research</source>
          <year>2018</year>
          <volume>37</volume>
          <fpage>1503912</fpage>
          <pub-id pub-id-type="doi">10.1080/17518369.2018.1503912</pub-id>
        </element-citation>
      </ref>
      <ref id="B11">
        <label>11.</label>
        <element-citation publication-type="book">
          <person-group person-group-type="author">
            <name><surname>Patin</surname><given-names>S.A.</given-names></name>
          </person-group>
          <source>Oil Spills and Their Impact on the Marine Environment and Living Resources</source>
          <publisher-loc>Moscow</publisher-loc>
          <publisher-name>Izd-vo VNIRO</publisher-name>
          <year>2008</year>
          <size units="pages">508</size>
          <comment>(in Russian)</comment>
        </element-citation>
      </ref>
      <ref id="B12">
        <label>12.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Voskoboinikov</surname><given-names>G.M.</given-names></name>
            <name><surname>Ryzhik</surname><given-names>I.V.</given-names></name>
            <name><surname>Salakhov</surname><given-names>D.O.</given-names></name>
            <name><surname>Metelkova</surname><given-names>L.O.</given-names></name>
            <name><surname>Zhakovskaya</surname><given-names>Z.A.</given-names></name>
            <name><surname>Lopushanskaya</surname><given-names>E.M.</given-names></name>
          </person-group>
          <article-title>Absorption and Conversion of Diesel Fuel by the Red Alga Palmaria palmata (Linnaeus) F. Weber et D. Mohr, 1805 (Rhodophyta): The Potential Role of Alga in Bioremediation of Sea Water</article-title>
          <source>Russian Journal of Marine Biology</source>
          <year>2020</year>
          <volume>46</volume>
          <issue>2</issue>
          <fpage>135</fpage>
          <lpage>141</lpage>
          <pub-id pub-id-type="doi">10.1134/S1063074020020108</pub-id>
        </element-citation>
      </ref>
      <ref id="B13">
        <label>13.</label>
        <element-citation publication-type="book">
          <person-group person-group-type="author">
            <name><surname>Voskoboinikov</surname><given-names>G.M.</given-names></name>
            <name><surname>Kamnev</surname><given-names>A.N.</given-names></name>
          </person-group>
          <source>Morphological and Functional Changes of Chloroplasts in the Algae Ontogenesis</source>
          <publisher-loc>Saint Petersburg</publisher-loc>
          <publisher-name>Nauka</publisher-name>
          <year>1991</year>
          <size units="pages">95</size>
          <comment>(in Russian)</comment>
        </element-citation>
      </ref>
      <ref id="B14">
        <label>14.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Voskoboinikov</surname><given-names>G.M.</given-names></name>
            <name><surname>Metelkova</surname><given-names>L.O.</given-names></name>
            <name><surname>Pugovkin</surname><given-names>D.</given-names></name>
            <name><surname>Salakhov</surname><given-names>D.</given-names></name>
          </person-group>
          <article-title>The Effect of Crude Oil on the Symbiotic Association of the Green Alga Acrosiphonia arcta (Dillwyn) Gain and Epiphytic Bacteria</article-title>
          <source>Marine Biological Journal</source>
          <year>2023</year>
          <volume>8</volume>
          <issue>1</issue>
          <fpage>16</fpage>
          <lpage>26</lpage>
        </element-citation>
      </ref>
      <ref id="B15">
        <label>15.</label>
        <element-citation publication-type="book">
          <person-group person-group-type="author">
            <name><surname>Mironov</surname><given-names>O.G.</given-names></name>
          </person-group>
          <source>Interaction Between Sea Organisms and Oil Hydrocarbons</source>
          <publisher-loc>Leningrad</publisher-loc>
          <publisher-name>Gidrometeoizdat</publisher-name>
          <year>1985</year>
          <size units="pages">127</size>
          <comment>(in Russian)</comment>
        </element-citation>
      </ref>
      <ref id="B16">
        <label>16.</label>
        <element-citation publication-type="journal">
          <person-group person-group-type="author">
            <name><surname>Binark</surname><given-names>N.</given-names></name>
            <name><surname>Güven</surname><given-names>K.C.</given-names></name>
            <name><surname>Gezgin</surname><given-names>T.</given-names></name>
            <name><surname>Ünlü</surname><given-names>S.</given-names></name>
          </person-group>
          <article-title>Oil Pollution of Marine Algae</article-title>
          <source>Bulletin of Environmental Contamination and Toxicology</source>
          <year>2000</year>
          <volume>64</volume>
          <fpage>866</fpage>
          <lpage>872</lpage>
          <pub-id pub-id-type="doi">10.1007/s001280000083</pub-id>
        </element-citation>
      </ref>
    </ref-list>
  </back>
</article>
