The paper studies the dynamics of biogenic elements (mineral (phosphate) and total phosphorus and ammonium) content based on the results of annual monitoring surveys of water in the eastern Gulf of Finland conducted in 2020-2022. Information on the horizontal and vertical distribution of the indicators was analysed, so samples were taken in the surface, bottom and middle (at deep-water stations) layers of water. The content of elements was determined by the spectrophotometric method. The results are compared and analysed by median values. During the study period, phosphate phosphorus concentrations in the absolute majority of cases did not exceed the maximum permissible concentration (0.15 mg/dm³), total phosphorus concentrations on average corresponded to the mesotrophic status, although there were cases of its concentration increase to values characteristic of the eutrophic status of a water body. Namely, in 2020, the concentrations amounted up to 0.091 mgP/dm³ in the bottom and surface water layers in June (mainly at the coastal stations) and in September (mainly in the bottom layer at the central offshore stations). In summer 2021, the concentrations reached 0.147 mgP/dm³ (surface layer) and 0.171 mgP/dm³ (bottom layer) at the coastal stations and 0.163 mgP/dm³ at the central station. Ammonia nitrogen concentrations were mainly within the MPC (0.5 mg/dm³). In June 2021, local areas along the southern and northern shores of the Gulf of Finland with relatively high levels of ammonia nitrogen (up to 0.285 mg/dm³) in surface and bottom water layers were identified. In general, despite the high anthropogenic load, concentrations of mineral phosphorus and ammonium in the waters of the Gulf of Finland were within the MPC, with exceedances recorded rarely, usually in Neva Bay, Koporye Bay and near the coast of the Kurortny district. Elevated concentrations of total phosphorus at the central stations can apparently be explained by transport of the substance from the western part of the Gulf and diffusion from bottom sediments. On average, higher concentrations of total phosphorus were found in bottom water layers than in surface water layers. In general, concentrations of biogenic elements correspond to the mesotrophic status of the water body.