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  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">Ecological Safety of Coastal and Shelf Zones of Sea</journal-id>
      <journal-title-group>
        <journal-title>Ecological Safety of Coastal and Shelf Zones of Sea</journal-title>
      </journal-title-group>
      <issn publication-format="print">2413-5577</issn>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">20240109</article-id>
      
      <article-categories>
        <subj-group subj-group-type="article-type">
          <subject>Research Article</subject>
        </subj-group>
      </article-categories>

      <title-group>
        <article-title>The Content of Hydrocarbons and Indicator Groups of Bacteria in the Marine Environment of Laspi Bay (Southern Coast of Crimea)</article-title>
      </title-group>

      <contrib-group>
        <contrib contrib-type="author">
          <name>
            <surname>Tikhonova</surname>
            <given-names>E. A.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>tihonoval@mail.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Soloveva</surname>
            <given-names>O. V.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>kozlya_oly@mail.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Tkachenko</surname>
            <given-names>Yu. S.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>yulechkatkachenko.90@mail.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Burdiyan</surname>
            <given-names>N. V.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>burdiyan@mail.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Doroshenko</surname>
            <given-names>Yu. V.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>julia_doroshenko@mail.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Guseva</surname>
            <given-names>E. V.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>guseva_ev@ibss-ras.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
        <contrib contrib-type="author">
          <name>
            <surname>Alyomov</surname>
            <given-names>S. V.</given-names>
          </name>
          <address>
            <country country="RU">Russian Federation</country>
          </address>
          <email>alyomov_sv@ibss-ras.ru</email>
          <xref ref-type="aff" rid="aff1"/>
        </contrib>
      </contrib-group>

      <aff id="aff1">
        <institution>A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS</institution>
        <addr-line>Sevastopol</addr-line>
        <country country="RU">Russian Federation</country>
      </aff>

      <pub-date date-type="pub" iso-8601-date="2024-03-25" publication-format="electronic">
        <day>25</day>
        <month>03</month>
        <year>2024</year>
      </pub-date>
      <volume>2024</volume>
      <issue>1</issue>
      <fpage>113</fpage>
      <lpage>129</lpage>

      <history>
        <date date-type="received" iso-8601-date="2023-11-30">
          <day>30</day>
          <month>11</month>
          <year>2023</year>
        </date>
        <date date-type="rev-recd" iso-8601-date="2023-12-27">
          <day>27</day>
          <month>12</month>
          <year>2023</year>
        </date>
        <date date-type="accepted" iso-8601-date="2023-12-10">
          <day>10</day>
          <month>12</month>
          <year>2023</year>
        </date>
      </history>

      <permissions>
        <copyright-statement>Copyright © 2024, E. A. Tikhonova, O. V. Soloveva, Yu. S. Tkachenko, N. V. Burdiyan, Yu. V. Doroshenko, E. V. Guseva, S. V. Alyomov</copyright-statement>
        <copyright-year>2024</copyright-year>
        <copyright-holder>E. A. Tikhonova, O. V. Soloveva, Yu. S. Tkachenko, N. V. Burdiyan, Yu. V. Doroshenko, E. V. Guseva, S. V. Alyomov</copyright-holder>
        <ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/>
        <license>
          <ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://creativecommons.org/licenses/by-nc/4.0/</ali:license_ref>
        </license>
      </permissions>

      <self-uri xlink:href="https://ecological-safety.ru/en/repository/issues/2024/01/09/" xlink:title="Page of article">https://ecological-safety.ru/en/repository/issues/2024/01/09/</self-uri>

      <abstract>
        <p>The paper assesses the quality of the marine environment of Laspi Bay near the Batiliman Stow according to main chemical and microbiological parameters under various recreational impacts on the water area. The material for the study was water and fouling samples taken in May, July and October 2023. The qualitative and quantitative composition of hydrocarbons was determined by gas chromatography on a Crystal 5000.2 chromatograph with a flame ionization detector in the Scientific and Educational Center for Collective Use «Spectrometry and Chromatography» of A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS. Diagnostic markers of the origin of hydrocarbons were used to identify possible sources of organic substances. The abundance of bacteria groups (saprophytic heterotrophic, hydrocarbon-oxidizing, lipolytic and phenol-oxidizing) was determined by the method of tenfold dilutions using elective nutrient media. From May to October 2023, the concentration of hydrocarbons in the coastal waters of the Batiliman Stow was 0.013-0.304 mg·L⁻¹. The composition of n-alkanes indicated the absence of oil pollution in the studied water area. The exceedance of the maximum permissible concentration for hydrocarbons, noted in July at one of the stations, is of natural origin and is associated with an active intake of allochthonous compounds. The quantitative assessment of the mentioned bacteria groups in the water and microperiphyton of macrofouling indicates an increase in the abundance of indicator groups of bacteria in all samples taken in July. Nevertheless, the results of the hydrocarbon content study and the quantitative assessment of the main microbiological indicators in the water and microperiphyton of macrofouling suggest that there are active bacterial self-purification processes in the water area of the Batiliman Stow. According to microbiological indicators, the studied area can be classified as conditionally clean.</p>
      </abstract>

      <kwd-group>
        <kwd>coastal water area</kwd>
        <kwd>recreational load</kwd>
        <kwd>seawater</kwd>
        <kwd>markers</kwd>
        <kwd>periphyton</kwd>
        <kwd>heterotrophic bacteria</kwd>
        <kwd>hydrocarbon-oxidizing bacteria</kwd>
        <kwd>lipolytic bacteria</kwd>
        <kwd>phenol-oxidizing bacteria</kwd>
        <kwd>macrophytes</kwd>
        <kwd>Laspi Bay</kwd>
        <kwd>anthropogenic pollution</kwd>
        <kwd>petroleum hydrocarbons</kwd>
      </kwd-group>

      <funding-group>
        <funding-statement>The work was carried out under IBSS state research assignment "Study of biogeochemical patterns of radioecological and chemoecological processes in the ecosystems of water bodies of the Sea of Azov–Black Sea Basin in comparison with other areas of the World Ocean and individual aquatic ecosystems of their drainage basins to ensure sustainable development in the southern seas of Russia" (no. 1023032000047-8-1.6.19).</funding-statement>
      </funding-group>

      <author-notes>
        <p><bold>About the authors:</bold></p>
        <p><bold>Elena A. Tikhonova</bold>, Leading Research Associate, A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2 Nakhimova Ave, Sevastopol, 299011, Russian Federation), PhD (Biol.), <bold>ORCID: 0000-0002-9137-087X</bold>, <bold>Scopus Author ID: 57208495804</bold>, <bold>ResearcherID: X-8524-2019</bold>, <email>tihonoval@mail.ru</email></p>
        <p><bold>Olga V. Soloveva</bold>, Leading Research Associate, A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2 Nakhimova Ave, Sevastopol, 299011, Russian Federation), PhD (Biol.), <bold>ORCID: 0000-0002-1283-4593</bold>, <bold>Scopus Author ID: 57208499211</bold>, <bold>ResearcherID: X-4793-2019</bold>, <email>kozlya_oly@mail.ru</email></p>
        <p><bold>Yulia S. Tkachenko</bold>, Junior Research Associate, A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2 Nakhimova Ave, Sevastopol, 299011, Russian Federation), <bold>ORCID: 0009-0001-1752-1043</bold>, <bold>Scopus Author ID: 1220495</bold>, <email>yulechkatkachenko.90@mail.ru</email></p>
        <p><bold>Nataliya V. Burdiyan</bold>, Senior Research Associate, A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2 Nakhimova Ave., Sevastopol, 299011, Russian Federation), PhD (Biol.), <bold>ORCID: 0000-0001-8030-1556</bold>, <bold>Scopus Author ID: 57208497483</bold>, <bold>ResearcherID: AAD-1704-2022</bold>, <email>burdiyan@mail.ru</email></p>
        <p><bold>Yulia V. Doroshenko</bold>, Research Associate, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2 Nakhimova Ave., Sevastopol, 299011, Russian Federation), PhD (Biol.), <bold>ORCID: 0000-0003-0498-3369</bold>, <bold>Scopus Author ID: 57211643141</bold>, <bold>ResearcherID: AAD-1706-2022</bold>, <email>julia_doroshenko@mail.ru</email></p>
        <p><bold>Elena V. Guseva</bold>, Junior Research Associate, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2 Nakhimova Ave., Sevastopol, 299011, Russian Federation), <bold>Scopus Author ID: 57208488324</bold>, <email>guseva_ev@ibss-ras.ru</email></p>
        <p><bold>Sergey V. Alyomov</bold>, Leading Research Associate, A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS (2 Nakhimova Ave., Sevastopol, 299011, Russian Federation), PhD (Biol.), <bold>ORCID: 0000-0002-3374-0027</bold>, <bold>Scopus Author ID: 24070027300</bold>, <email>alyomov_sv@ibss-ras.ru</email></p>
        <p><bold>Contribution of the authors:</bold></p>
        <p><bold>Elena A. Tikhonova</bold> – statement of aims and objectives of the complex study, water and fouling sampling, manuscript writing</p>
        <p><bold>Olga V. Soloveva</bold> – analysis of the results obtained on the hydrocarbon composition of water, results discussion, manuscript writing</p>
        <p><bold>Yulia S. Tkachenko</bold> – preparation of water samples, determination of qualitative and quantitative composition of hydrocarbons in the water, writing and presentation of the article</p>
        <p><bold>Nataliya V. Burdiyan</bold> – statement of the objectives of the microbiological study, analysis of the results obtained in the microbiological study, results discussion, manuscript writing, microbiological works to determine the abundance of indicator groups of bacteria in the periphyton</p>
        <p><bold>Yulia V. Doroshenko</bold> – analysis of the obtained results on microbiological research, results discussion, manuscript writing, microbiological works to determine the abundance of indicator groups of bacteria in the water</p>
        <p><bold>Elena V. Guseva</bold> – study rationale, preparation of the literature review, fouling sampling, construction of graphical materials</p>
        <p><bold>Sergey V. Alyomov</bold> – sampling, description of the macrofouling community and its changes during different sampling periods</p>
        <p><italic>All the authors have read and approved the final manuscript.</italic></p>
      </author-notes>
    </article-meta>
  </front>

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