K. M. Zaripova^1,*, E. A. Demidova², E. A. Tikhonova¹, N. V. Burdiyan¹, Yu. V. Doroshenko¹, E. D. Basova³

¹ A. O. Kovalevsky Institute of Biology of the Southern Seas of Russian Academy of Sciences, Sevastopol, Russian Federation

² Moscow Institute of Physics and Technology, Dolgoprudny, Russian Federation

³ Institute of Oil and Gas Problems of the Russian Academy of Sciences, Moscow, Russian Federation

^* e-mail: zaripova_km@ibss-ras.ru

Abstract

The paper presents the results of microbiological study in the coastal waters of the northwestern Pacific Ocean and the Sea of Okhotsk, obtained during the expedition of cruise 23/4 of PV Professor Multanovsky (August–September 2023) within the Floating University Program. Quantitative characteristics and spatial distribution of bacteria transforming major classes of organic compounds, including petroleum hydrocarbons (diesel fuel), lipids, and phenols, in the surface and bottom layers of the water column were determined. The abundance of heterotrophic bacteria in the surface and bottom layers ranged from 10³ to 10⁵ cells/mL. The abundance of hydrocarbon-oxidizing bacteria ranged from 1 to 10² cells/mL in the surface layer and from 1 to 10 cells/mL in the bottom layer. The abundance of lipolytic bacteria varied from 10 to 10³ cells/mL in both layers. The phenol-oxidizing bacterial group ranged in abundance values from 1 to 10² cells/mL in both the surface and bottom layers. The investigated bacterial groups exhibited non-uniform distribution in the coastal waters of the Kamchatka Peninsula. Maximum values are associated with the anthropogenically loaded Avacha Bay, exploited bays, and sites of active river runoff. During the study period, the water temperature of the surface layer ranged from 11.0 to 16.1°C, and the bottom layer ranged from 1.3 to 11.3°C. The pH values in surface water varied from 8.38 to 8.49. The depth at the bottom water sampling stations was from 13.5 to 780 m in the Pacific Ocean and from 26 to 62 m in the Sea of Okhotsk. No significant correlation was found between the abundance of identified microbial groups and the recorded physical and chemical parameters.

Keywords

heterotrophic bacteria, coastal waters, Kamchatka Peninsula, Avacha Bay, Sea of Okhotsk

Acknowledgments

The work was carried out during cruise 23/4 of PV Professor Multanovsky as part of the Floating University Program (Agreement no. 075-01593-23-06) with the support of the Ministry of Science and Higher Education of the Russian Federation. The nutrient media for determining the abundance of indicator groups of bacteria were prepared as part of the IBSS state research assignment “Study of biogeochemical patterns of radioecological and chemoecological processes in the ecosystems of water bodies of the Sea of Azov–Black Sea Basin in comparison with other areas of the World Ocean and individual aquatic ecosystems of their drainage basins to ensure sustainable development in the southern seas of Russia” (No. 124030100127-7).

For citation

Zaripova, K.M., Demidova, E.A., Tikhonova, E.A., Burdiyan, N.V., Doroshenko, Yu.V. and Basova, E.D., 2025. The Abundance and Distribution of Individual Bacterial Groups in Coastal Waters of the Kamchatka Peninsula. Ecological Safety of Coastal and Shelf Zones of Sea, (2), pp. 135–148.

References

1. Pandolfo, E., Caracciolo, A.B. and Rolando, L., 2023. Recent Advances in Bacterial Degradation of Hydrocarbons. Water, 15(2), 375. https://doi.org/10.3390/w15020375
2. Giri, K. and Rai, J.P.N., 2014. Bacterial Metabolism of Petroleum Hydrocarbons. In: M. Ahmad, ed., 2014. Biotechnology. Vol. 11: Biodegradation and Bioremediation. Studium Press India Pvt. Ltd., pp. 73–93.
3. Abdullah, S.R.S., Al-Baldawi, I.A., Almansoory, A.F., Purwanti, I.F., Al-Sbani, N.H. and Sharuddin, S.S.N., 2020. Plant-Assisted Remediation of Hydrocarbons in Water and Soil: Application, Mechanisms, Challenges and Opportunities. Chemosphere, 247, 125932. https://doi.org/10.1016/j.chemosphere.2020.125932
4. Nemirovskaya, I.A., 2013. Oil in the Ocean (Pollution and Natural Flow). Moscow: Nauchny Mir, 428 p. (in Russian).
5. Mironov, O.G., 2002. Bacterial Transformation of Oil Hydrocarbons in the Coastal Zone of Sea. Marine Ekological Journal, 1(1), pp. 56–66. (in Russian).
6. Trudnev, S.Yu. and Nistor, A.S., 2020. The Analysis of Accident Rate of Fishing Fleet Vessels in the Far East. In: O. A. Belov, ed., 2020. [Technical Operation of Water Transport: Problems and Ways of Development. Proceedings of the Second International Scientific and Technical Conference (23–25 October 2019)]. Petropavlovsk-Kamchatsky: KamchatGTU, pp. 66–69 (in Russian).
7. Shevtsov, M.N. and Makarova, V.S., 2023. Assessment of Possible Negative Consequences from Accidental Spill of Oil Products on the Sea Area during Cargo Transfer from the Barge Operations. Bulletin of PNU, (1), pp. 153–160 (in Russian).
8. Zhuravel, E.V., Bezverbnaya, I.P. and Buzoleva, L.S., 2004. Microbian Indication of Pollution of the Coastal Zone of the Sea of Okhotsk and Avacha Bay. Russian Journal of Marine Biology, 30(2), pp. 138–142. (in Russian).
9. Dimitrieva, G.Yu., Bezverbnaya, I.P. and Khristoforova, N.K., 2001. Microbial Indication as Possible Approach for Heavy Metals Monitoring in Far-Eastern Seas. Izvestia TINRO, 128(3), pp. 719–736. (in Russian).
10. Dimitrieva, G.Yu. and Bezverbnaya, I.P., 2002. Microbial Indication as Effective Tool for Monitoring of Heavy Metals Pollution of Coastal Sea Waters. Okeanologiya, 42(3), pp. 408–415 (in Russian).
11. Sergeenko, N.V. and Ustimenko, E.A., 2016. In: A. V. Gaevskaya, ed., 2016. Marine Biological Research: Achievements and Perspectives. Proceedings of All-Russian Scientific-Practical Conference with International Participation Dedicated to the 145^th Anniversary of Sevastopol Biological Station (Sevastopol, 19–24 September, 2016). Sevastopol: EKOSI-Gidrofizika, Vol. 3, pp. 214–217 (in Russian).
12. Koneva, M.N. and Stupnikova, N.A., 2021. Oil-Oxidizing Microorganisms as Indicators of Oil Pollution of Watercourses of Petropavlovsk-Kamchatsky. International Research Journal, (7), pp. 23–27 (in Russian).
13. Sanamyan, N.P., Korobok, A.V. and Sanamyan, K.E., 2023. Qualitative Assessment of Algae Harmful Bloom Impact in Autumn 2020 off the Coast of South-Eastern Kamchatka (North-West Pacific) on Shallow-Water Benthic Communities. Bulletin of Kamchatka State Technical University, (63), pp. 22–43 (in Russian).
14. The Mysterious Mass Death of Marine Organisms on the Kamchatka Peninsula: A Consequence of a Technogenic Impact on the Environment or a Natural Phenomenon? Marine Pollution Bulletin, 166, 112175. https://doi.org/10.1016/j.marpolbul.2021.112175
15. Voroshilova, A.A. and Dianova, E.V., 1952. [Oil-Oxidizing Bacteria – Performance Indices of Oil Biological Oxidation under Natural Conditions]. Mikrobiologiya, 21(4), pp. 408–415 (in Russian).
16. Ermolaev, K.K. and Mironov, O.G., 1975. The Role of Phenol Decomposing Microorganisms in Detoxication of Phenol in the Black Sea. Mikrobiologiya, 10(5), pp. 928–932 (in Russian).
17. Chalov, S.R., Tsyplenkov, A.S., Shkolny, D.I., Prokopeva, K.N. and Bahareva, E.I., 2022. Overland Runoff and Its Impact on Hydrobiont Mortality in Avachinsky Gulf (Pacific Ocean, Kamchatka). Proceedings of the Russian Geographical Society, 154(4), pp. 69–84. https://doi.org/10.31857/S0869607122040048 (in Russian).
18. Rusanova, V.A. and Sedova N.A., 2023. Determining the Content of Oil Products in Water and Bottom Sediments of Avacha Bay in 2022. In: E. G. Lobkov, 2023. [Natural Resources, Their Current State, Protection, Commercial and Technical Application : Proceedings of the 14^th National (All-Russian) Scientific and Practical Conference (21–22 March 2023)]. Petropavlovsk-Kamchatsky: KamchatGTU, pp. 149–152. https://doi.org/10.24412/cl-35030-2023-1-149-152
19. Chaudhry, G.R., ed., 1994. Biological Degradation and Bioremediation of Toxic Chemicals. Oregon: Dioscorides Press, 1994. 515 p.

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